Coding

Part:BBa_K4739008:Design

Designed by: Huaiyuan Ma   Group: iGEM23_SUSTech-Shenzhen   (2023-10-10)


VgrG3-Tenecteplase fusion protein


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2547
    Illegal EcoRI site found at 3019
    Illegal XbaI site found at 3517
    Illegal PstI site found at 3136
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2547
    Illegal EcoRI site found at 3019
    Illegal PstI site found at 3136
    Illegal NotI site found at 2818
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2547
    Illegal EcoRI site found at 3019
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2547
    Illegal EcoRI site found at 3019
    Illegal XbaI site found at 3517
    Illegal PstI site found at 3136
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2547
    Illegal EcoRI site found at 3019
    Illegal XbaI site found at 3517
    Illegal PstI site found at 3136
    Illegal NgoMIV site found at 3574
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1. Choose codons that are frequently used in the host organism to ensure efficient translation. 2. Optimize for codons corresponding to abundant tRNAs in the host to enhance translation speed. 3. Limit repetitive DNA sequences that may lead to recombination events and genomic instability.


Source

Sequence of VgrG3 gene is from V. cholerae genome, sequence of Dulaglutide is built from its amino acid sequence.

References