Coding

Part:BBa_K4739006:Design

Designed by: Huaiyuan Ma   Group: iGEM23_SUSTech-Shenzhen   (2023-10-10)


VgrG3-KLA fusion protein


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1978
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1947
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1978
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1978
    Illegal NgoMIV site found at 2035
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1. Choose codons that are frequently used in the host organism to ensure efficient translation. 2. Optimize for codons corresponding to abundant tRNAs in the host to enhance translation speed. 3. Limit repetitive DNA sequences that may lead to recombination events and genomic instability.



Source

Sequence of VgrG3 gene is from V. cholerae genome, sequence of KLA is built from its amino acid sequence.

References