Coding
Part:BBa_K4739004:Design
Designed by: Huaiyuan Ma Group: iGEM23_SUSTech-Shenzhen (2023-10-09)
Hcp-Minihepcidin fusion protein
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 487
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 142
Illegal NgoMIV site found at 265 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
1. Choose codons that are frequently used in the host organism to ensure efficient translation. 2. Optimize for codons corresponding to abundant tRNAs in the host to enhance translation speed. 3. Limit repetitive DNA sequences that may lead to recombination events and genomic instability.
Source
Sequence of the linker is retrieved from a research. The sequence of Hcp is retrieved from P. aeruginosa genome. The sequence of Minihepcidin is created from its amino acid sequence.
References
Jana, B., Keppel, K., & Salomon, D. (2021). Engineering a customizable antibacterial T6SS‐based platform in Vibrio natriegens. EMBO Reports. https://doi.org/10.15252/embr.202153681