Part:BBa_K4737014:Design

pnirB-SopE-Flag-GOx

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

None.

Source

The BNUZH-China iGEM team 2023 designed and built.

References

1.Liu Z, Liu X, Cao W, Hua ZC. Tumor-specifically hypoxia-induced therapy of SPRY1/2 displayed differential therapeutic efficacy for melanoma. Am J Cancer Res. 2015 Jan 15;5(2):792-801. PMID: 25973316; PMCID: PMC4396039.
2.Kriechbaum M, Heilmann HJ, Wientjes FJ, Hahn M, Jany KD, Gassen HG, Sharif F, Alaeddinoglu G. Cloning and DNA sequence analysis of the glucose oxidase gene from Aspergillus niger NRRL-3. FEBS Lett. 1989 Sep 11;255(1):63-6. doi: 10.1016/0014-5793(89)81061-0. PMID: 2792372.
3.Jayaraman PS, Gaston KL, Cole JA, Busby SJ. The nirB promoter of Escherichia coli: location of nucleotide sequences essential for regulation by oxygen, the FNR protein and nitrite. Mol Microbiol. 1988 Jul;2(4):527-30. doi: 10.1111/j.1365-2958.1988.tb00059.x. PMID: 2845227.