Composite

Part:BBa_K4737013:Design

Designed by: Jintao Qiu   Group: iGEM23_BNUZH-China   (2023-08-27)


prpsM::Lpp-OmpA-scFv


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1
    Illegal PstI site found at 34
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1
    Illegal PstI site found at 34
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1
    Illegal PstI site found at 34
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None.

Source

The BNUZH-China iGEM team 2023 designed and built.

References

1.Movva NR, Nakamura K, Inouye M. Regulatory region of the gene for the ompA protein, a major outer membrane protein of Escherichia coli. Proc Natl Acad Sci U S A. 1980 Jul;77(7):3845-9. doi: 10.1073/pnas.77.7.3845. PMID: 7001445; PMCID: PMC349723.
2.Francisco, J. A., Campbell, R., Iverson, B. L. & Georgiou, G. Production and fluorescence-activated cell sorting of Escherichia coli expressing a functional antibody fragment on the external surface. Proc Natl Acad Sci U S A 90, 10444-10448 (1993). https://doi.org:10.1073/pnas.90.22.10444
3.Marshall, J. Carcinoembryonic antigen-based vaccines. Semin Oncol 30, 30-36 (2003). https://doi.org:10.1016/s0093-7754(03)00233-1