DNA

Part:BBa_K4737008:Design

Designed by: Jintao Qiu   Group: iGEM23_BNUZH-China   (2023-08-27)


HlyA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We selected HlyA, the gene encoding Listeriolysin-O protein from Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e), for our experiment.

Source

The source of the part will be its sequence which was retrieved from GenBank.

References

Domann E, Chakraborty T. Nucleotide sequence of the listeriolysin gene from a Listeria monocytogenes serotype 1/2a strain. Nucleic Acids Res. 1989 Aug 11;17(15):6406. doi: 10.1093/nar/17.15.6406. PMID: 2505236; PMCID: PMC318305.