Primer

Part:BBa_K4734002:Design

Designed by: Srika Popuri   Group: iGEM23_RBHS-SanDiego-CA   (2023-10-11)


Reverse Primer for Linearizing Vector


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

One design consideration we had to deal with was finding the place to design our primers. We needed to be very accurate when placing our primers in the correct location because it was crucial for inserting our epitope into the phage so that it is displayed.


Source

The plasmid was designed by our team by using the M13KE phage sequence. It is placed exactly at the point that we need it to be, so that it can linearize our vector to insert our epitope.

References