Plasmid
Part:BBa_K4730005
Designed by: Sanvi Garg Group: iGEM23_WVHS-SanDiego (2023-10-09)
Level 1 MHETase plasmid
This is the level 1 MHETase plasmid that we created with an inducible rhamnose promoter, sfGFP, and EF-rrnB terminator along with the MHEtase gene sequence. We were able to successfully combine this with our level 1 PETase plasmid and conjugate our combined level 2 plasmid into alteromonas.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1175
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 3121
Illegal AgeI site found at 1010
Illegal AgeI site found at 2341
Illegal AgeI site found at 2458 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 992
Illegal SapI.rc site found at 1291
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Categories
Parameters
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