Plasmid

Part:BBa_K4730005

Designed by: Sanvi Garg   Group: iGEM23_WVHS-SanDiego   (2023-10-09)


Level 1 MHETase plasmid

This is the level 1 MHETase plasmid that we created with an inducible rhamnose promoter, sfGFP, and EF-rrnB terminator along with the MHEtase gene sequence. We were able to successfully combine this with our level 1 PETase plasmid and conjugate our combined level 2 plasmid into alteromonas.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1175
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3121
    Illegal AgeI site found at 1010
    Illegal AgeI site found at 2341
    Illegal AgeI site found at 2458
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 992
    Illegal SapI.rc site found at 1291


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Parameters
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