Part:BBa_K4730002:Design
Level 1 PETase plasmid
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1175
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1010
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 992
Illegal SapI.rc site found at 1291
Design Notes
Our original terminator was degraded in the golden gate assembly machine so we had to change it from B0015 terminator to the EF-rrnB terminator.
Source
ATG AAT TTC CCC CGT GCA TCT CGC TTG ATG CAG GCA GCA GTA CTG GGG GGC CTG ATG GCA GTA TCG GCA GCT GCT ACA GCC CAG ACG AAT CCA TAT GCT CGT GGC CCA AAT CCG ACC GCT GCA AGC CTG GAA GCG AGT GCA GGC CCT TTT ACA GTC CGT AGT TTC ACG GTG TCT CGT CCC AGC GGT TAC GGA GCA GGG ACA GTA TAC TAT CCC ACC AAC GCT GGC GGG ACC GTT GGG GCG ATT GCC ATT GTT CCG GGG TAC ACG GCT CGT CAA TCG AGC ATT AAG TGG TGG GGT CCC CGC CTT GCT TCA CAC GGC TTC GTC GTC ATT ACC ATT GAC ACA AAT TCT ACA CTG GAT CAG CCA AGC TCA CGC TCC TCT CAA CAA ATG GCG GCT TTG CGT CAA GTT GCC TCA CTT AAC GGC ACG TCA TCC TCC CCC ATC TAC GGT AAA GTT GAC ACG GCT CGT ATG GGC GTT ATG GGT TGG TCA ATG GGA GGA GGA GGT TCA TTA ATT TCA GCC GCT AAT AAC CCG TCG CTG AAG GCC GCC GCT CCT CAA GCA CCG TGG GAC TCC TCA ACG AAC TTC TCA AGC GTA ACT GTA CCT ACG TTA ATT TTT GCT TGT GAA AAT GAT AGT ATC GCT CCG GTC AAC TCC TCG GCG CTG CCC ATT TAC GAC TCA ATG AGC CGC AAT GCA AAG CAA TTT TTA GAG ATT AAT GGT GGA TCA CAC AGT TGC GCG AAC AGC GGA AAC TCA AAC CAG GCT TTG ATC GGG AAG AAA GGG GTA GCA TGG ATG AAA CGC TTC ATG GAC AAC GAT ACT CGT TAC TCT ACT TTT GCC TGC GAG AAC CCT AAC TCC ACA CGC GTG TCC GAC TTC CGT ACA GCA AAT TGT TCC