Reporter

Part:BBa_K4729204

Designed by: Melina Flakowski   Group: iGEM23_Marburg   (2023-09-04)


mNeonGreen (A. tumefaciens optimized)

Reporter genes are genetic tools that serve as vital indicators of transcriptional activity within cells. These exogenous coding regions are linked to promoter sequences and introduced into cells, allowing scientists to measure promoter activity. Once expressed, reporters are assessed by measuring either the reporter protein or its enzymatic activity, providing a direct correlation between promoter strength and reporter production.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 179
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 36
  • 1000
    COMPATIBLE WITH RFC[1000]

References

taken from:

iGEM TU Eindhoven 2015
BBa_K1761003 and codon adapted for A. rhizogenes


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