Part:BBa_K4729202

StayGold (A. tumefaciens optimized)

Reporter genes are genetic tools that serve as vital indicators of transcriptional activity within cells. These exogenous coding regions are linked to promoter sequences and introduced into cells, allowing scientists to measure promoter activity. Once expressed, reporters are assessed by measuring either the reporter protein or its enzymatic activity, providing a direct correlation between promoter strength and reporter production.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 563
  Illegal XhoI site found at 634
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

taken from:

Hirano, M., Ando, R., Shimozono, S., Sugiyama, M., Takeda, N., Kurokawa, H., Deguchi, R., Endo, K., Haga, K., Takai-Todaka, R., Inaura, S., Matsumura, Y., Hama, H., Okada, Y., Fujiwara, T., Morimoto, T., Katayama, K., & Miyawaki, A. (2022). A highly photostable and bright green fluorescent protein. In Nature Biotechnology (Vol. 40, Issue 7, pp. 1132–1142). Springer Science and Business Media LLC. https://doi.org/10.1038/s41587-022-01278-2

and codon optimized for A. rhizogenes