Composite

Part:BBa_K4728018:Design

Designed by: Bryant Han   Group: iGEM23_Queens-Canada   (2023-10-10)


Constitutive TphR expression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A strong RBS was chosen as well as a strong terminator. Various transcriptional units with different promoters constitutively expressing fluorescent proteins were assembled before deciding on BBa_J23100 for its strength.


Source

The CDS (BBa_K4728001) comes from Comamonas testosteroni, and all other parts from the 2023 iGEM distribution kit.

References