Coding

Part:BBa_K4728008:Design

Designed by: Igor Serafini   Group: iGEM23_Queens-Canada   (2023-10-12)


PhaF mutant


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 657
    Illegal NgoMIV site found at 672
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Following the truncation of the protein, a carboxyl group was introduced at its C-terminal end to enhance stability during our molecular dynamic simulations. This modification was crucial to ensure reliable and accurate simulations of the truncated protein. The sequence is codon optimized to Escherichia coli and is compatible with RFC[1000] and RFC[10] standards.


Source

The part is a modified version of PhaF, which comes from the genome of Pseudomonas putida

References

Maestro, Beatriz, et al. “A New Family of Intrinsically Disordered Proteins: Structural Characterization of the Major Phasin PhaF from Pseudomonas Putida KT2440.” PLoS ONE, vol. 8, no. 2, 15 Feb. 2013, p. e56904, https://doi.org/10.1371/journal.pone.0056904. Accessed 7 Feb. 2023.

Jendrossek, Dieter, and Daniel Pfeiffer. “New Insights in the Formation of Polyhydroxyalkanoate Granules (Carbonosomes) and Novel Functions of Poly(3-Hydroxybutyrate).” Environmental Microbiology, vol. 16, no. 8, 21 Jan. 2014, pp. 2357–2373, https://doi.org/10.1111/1462-2920.12356. Accessed 18 Nov. 2020.