Coding

Part:BBa_K4725000:Design

Designed by: Fung Ching Nam   Group: iGEM23_HKUST   (2023-10-11)


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Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The promoter can be fused with fluorescent protein for reporter assay. This promoter is leaky in E. coli. It is suggested not to use it in E. coli unless there is a specific reason.


Source

The sequence of this promoter is extracted from ‘Negative Interplay between Biofilm Formation and Competence in the Environmental Strains of Bacillus subtilis’ https://journals.asm.org/doi/10.1128/msystems.00539-20


References