Composite

Part:BBa_K4724076:Design

Designed by: Xinyao Li   Group: iGEM23_NJTech-China-A   (2023-10-07)


TrxA-LSPETase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 25


Design Notes

A recombinant plasmid constructed using the Gibson assembly method was inserted into the NusA gene sequence into vector pET-22b(+)-LSPET-12


Source

from E.coli

References