Composite
Part:BBa_K4724028:Design
Designed by: Qingqing Zheng Group: iGEM23_NJTech-China-A (2023-10-10)
IsPETaseQ119F/W159H-Linker-CBM11-6xHis Tag
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 56
Illegal PstI site found at 913 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 56
Illegal PstI site found at 913 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1134
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 56
Illegal PstI site found at 913 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 56
Illegal PstI site found at 913
Illegal AgeI site found at 546
Illegal AgeI site found at 926 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We needed to purify the IsPETase protein, so we attached the 6xHis tag after IsPETase.
Source
This is a new section that is followed by a domain in the IsPETase double mutant.