Composite

Part:BBa_K4724025:Design

Designed by: Qingqing Zheng   Group: iGEM23_NJTech-China-A   (2023-10-10)


IsPETase-Linker- LSChi5CBM -6xHisTag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 56
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 56
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 790
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 56
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 56
    Illegal NgoMIV site found at 890
    Illegal AgeI site found at 546
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use Linker to fuse the domain to the IsPETase protein and connect 6xHis Tag behind the domain for easy purification.


Source

Designed by NJTech-China-A.

References