Protein_Domain

Part:BBa_K4724000:Design

Designed by: Qingqing Zheng   Group: iGEM23_NJTech-China-A   (2023-10-06)


CBM3-6xHis Tag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 70
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 70
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 448
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 70
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 70
    Illegal NgoMIV site found at 28
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

You must use a flexible amino acid chain long enough to link it with protein.


Source

It's from Bacillus subtilis.

References

[1] Hwang, D.-H. et al. Enhanced biodegradation of waste poly(ethylene terephthalate) using a reinforced plastic degrading enzyme complex. Science of The Total Environment 842, 156890 (2022).

[2] Zhang, Y. et al. Interactions between type A carbohydrate binding modules and cellulose studied with a quartz crystal microbalance with dissipation monitoring. Cellulose 27, 3661–3675 (2020)