Part:BBa_K4724000
CBM3-6xHis Tag
CBM3 can be used to enhance the hydrophobicity of enzyme [1], thereby enhancing the binding ability and affinity between the enzyme and the substrate, and ultimately achieving the purpose of improving enzyme activity. The CBM3 domain usually has flexibility and variability [2], enabling it to adapt to the structural diversity of different substrates. It can adapt to different types of substrates through structural adjustment and closely bind to them. This adaptability and specificity help to enhance the binding ability and affinity between CBM3 and the substrate. This binding allows IsPETase to be closer to PET and bind to it, thereby increasing enzyme activity. In view of the fact that much literature has reported that CBM3 can promote the binding of enzymes to hydrophobic substrates, we chose CBM3 as our basic element to modify IsPETase to improve its binding ability and catalytic efficiency with the substrate PET.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 70
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 70
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 448
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 70
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 70
Illegal NgoMIV site found at 28 - 1000COMPATIBLE WITH RFC[1000]
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