Regulatory
Part:BBa_K4721011:Design
Designed by: Celeste Bos Group: iGEM23_Leiden (2023-10-11)
PV10
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4
Illegal BamHI site found at 43 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The -10 box of the strong Psyn2 was changed in order to accomodate a vanO operator site
Source
Synthetic vanillate inducible promoter designed by Julia Vorholt [DOI: 10.1038/srep06453]