Regulatory

Part:BBa_K4721011:Design

Designed by: Celeste Bos   Group: iGEM23_Leiden   (2023-10-11)


PV10


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
    Illegal BamHI site found at 43
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The -10 box of the strong Psyn2 was changed in order to accomodate a vanO operator site


Source

Synthetic vanillate inducible promoter designed by Julia Vorholt [DOI: 10.1038/srep06453]

References