Regulatory

Part:BBa_K4721008:Design

Designed by: Celeste Bos   Group: iGEM23_Leiden   (2023-10-11)


PV10 vanillate inducible system


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 862
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 752
    Illegal SpeI site found at 862
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 814
    Illegal BamHI site found at 853
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 862
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 862
    Illegal NgoMIV site found at 41
    Illegal NgoMIV site found at 198
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 422
    Illegal BsaI site found at 741
    Illegal BsaI.rc site found at 138
    Illegal SapI.rc site found at 688


Design Notes

None


Source

Part of pVYD plasmid from Julia Vorholt (Addgene plasmid # 61302 ; [http://n2t.net/addgene:61302] ; RRID:Addgene_61302)


References