Part:BBa_K4721006:Design
PL/O4/A1 IPTG inducible system
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 4
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 90
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 4
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 4
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The basic part for the lacIq promoter also contains a spacer sequence, after which the PL/O4/A1 part was placed
Source
This composite part is comprised of four basic parts, the lacIq repressor gene and its terminator, which is expressed by the lacIq promoter, and the PL/O4/A1, containing the lacO operator sequence. The part was designed by Carrillo et al. (2019)[1]
References
[1] Carrillo M, Wagner M, Petit F, Dransfeld A, Becker A, Erb TJ. Design and Control of Extrachromosomal Elements in Methylorubrum extorquens AM1. ACS Synth Biol. 2019;8(11):2451-2456. doi:10.1021/acssynbio.9b00220
[2] Schada Von Borzyskowski L, Remus-Emsermann M, Weishaupt R, Vorholt JA, Erb TJ. A set of versatile brick vectors and promoters for the assembly, expression, and integration of synthetic operons in methylobacterium extorquens am1 and other alphaproteobacteria. ACS Synth Biol. 2015;4(4):430-443. doi:10.1021/sb500221v
[3] Peyraud R, Kiefer P, Christen P, Massou S, Portais JC, Vorholt JA. Demonstration of the ethylmalonyl-CoA pathway by using13C metabolomics. Proc Natl Acad Sci U S A. 2009;106(12):4846-4851. doi:10.1073/pnas.0810932106
[4] Leveau JHJ, Lindow SE. Predictive and Interpretive Simulation of Green Fluorescent Protein Expression in Reporter Bacteria. J Bacteriol. 2001;183(23):6752-6762. doi:10.1128/JB.183.23.6752-6762.2001