![](https://parts.igem.org/images/partbypart/icon_regulatory.png)
Part:BBa_K4721004:Design
PL/O4/A1
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 4
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 4
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 4
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The upstream region was included to facilitate primer hybridization.
Source
PL/O4/A1 is a synthetic promoter designed by Carrillo et al. (2019)[1]. In order to function as an inducible promoter, the lacI gene with its respective lacIq promoter is expressed, which expresses the lac repressor, LacI. This repressor binds the _lacO _sequence, present in the PL/04/A1 in the interspacer region between the -10 and -33 sites. When Isopropyl β-d-1-thiogalactopyranoside (IPTG) is present, this repressor is released, and the respective gene (here mCherry) is expressed.
References
[1] Carrillo M, Wagner M, Petit F, Dransfeld A, Becker A, Erb TJ. Design and Control of Extrachromosomal Elements in Methylorubrum extorquens AM1. ACS Synth Biol. 2019;8(11):2451-2456. doi:10.1021/acssynbio.9b00220