Part:BBa_K4721003:Design

Ptuf

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 41
  Illegal PstI site found at 36
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 41
  Illegal PstI site found at 36
  Illegal NotI site found at 13
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 41
  Illegal XhoI site found at 8
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 41
  Illegal PstI site found at 36
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 41
  Illegal PstI site found at 36
  Illegal NgoMIV site found at 178
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The range of the Ptuf promoter sequence as decribed by Schada Von Borzyskowski et al. (2015) is only part of this submitted sequence. There is a scar, which is an artefact from the cloning process. The results were obtained with this scar included, therefore the part is sumbitted including this cloning artefact

Source

This part has been described by Schada Von Borzyskowski et al. (2015). The source of this part is the putative promoter region of EF-Tu, coding for the translation elongation factor thermo-unstable in M. extorquens AM1.

References

[1] Schada Von Borzyskowski L, Remus-Emsermann M, Weishaupt R, Vorholt JA, Erb TJ. A set of versatile brick vectors and promoters for the assembly, expression, and integration of synthetic operons in methylobacterium extorquens am1 and other alphaproteobacteria. ACS Synth Biol. 2015;4(4):430-443. doi:10.1021/sb500221v

[2] Peyraud R, Kiefer P, Christen P, Massou S, Portais JC, Vorholt JA. Demonstration of the ethylmalonyl-CoA pathway by using13C metabolomics. Proc Natl Acad Sci U S A. 2009;106(12):4846-4851. doi:10.1073/pnas.0810932106

[3] Leveau JHJ, Lindow SE. Predictive and Interpretive Simulation of Green Fluorescent Protein Expression in Reporter Bacteria. J Bacteriol. 2001;183(23):6752-6762. doi:10.1128/JB.183.23.6752-6762.2001