Primer
Part:BBa_K4717002:Design
Designed by: Jun Zhou Group: iGEM23_BIBS-beijing-china (2023-10-10)
LDHA primer
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The result by Primer3 using 1-based sequence positions: OLIGO start len tm gc% any 3' rep seq LEFT 17201 20 59.99 45.00 6.00 0.00 10.00 AGGCCAACTCTGCAAAGAAA RIGHT 17445 20 69.94 40.00 6.00 0.00 12.00 GCACCTGGCCAAAATAAAAA SEQUENCE SIZE: 18021 INCLUDED REGION SIZE: 18021 PRODUCT SIZE: 245, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 0.00
Source
In this project, we used Primer3 (https://bioinfo.ut.ee/primer3-0.4.0/) to design the primer sequence based on information of NCBI nucleoside database.