Primer

Part:BBa_K4717000:Design

Designed by: Jun Zhou   Group: iGEM23_BIBS-beijing-china   (2023-10-10)


EIF6 primer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The result by Primer3 using 1-based sequence positions: OLIGO start len tm gc% any 3' rep seq LEFT 1267 20 59.94 55.00 3.00 1.00 10.00 TCTCATCTCTCCCTGCCACT RIGHT 1510 20 60.00 50.00 3.00 0.00 10.00 GCAACAAGAGCGAAAACTCC SEQUENCE SIZE: 5867 INCLUDED REGION SIZE: 5867 PRODUCT SIZE: 244, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00


Source

In this project, we used Primer3 (https://bioinfo.ut.ee/primer3-0.4.0/) to design the primer sequence based on information of NCBI nucleoside database.

References