Regulatory

Part:BBa_K4716997:Design

Designed by: LIAO WENYU   Group: iGEM23_UM-Macau   (2023-10-10)


PnorV promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is the promoter that can bind with the regulator protein NorR, which is also an essential part of NO sensor.


Source

The sequence of this gene is directly taken from the following literature's supplementary materials. Reference: Xiaoyu J. Chen et al., (2021) Rational Design and Characterization of Nitric Oxide Biosensors in E. coli Nissle 1917 and Mini SimCells, ACS Synthetic Biology 2021, 10 (10), 2566-2578. doi: 10.1021/acssynbio.1c00223

References