Plasmid

Part:BBa_K4716991:Design

Designed by: LIAO WENYU   Group: iGEM23_UM-Macau   (2023-10-10)


PET-NorR-ProA-PnorV-pep-his tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 3792
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3038
    Illegal XhoI site found at 3801
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2027
    Illegal AgeI site found at 1030
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 61
    Illegal BsaI.rc site found at 3159
    Illegal SapI site found at 1254


Design Notes

First it is the material selection priorities, for us this release module we firstly use the vector we already have to directly use. Second we consider to use different antibiotic resistance gene in our two different core functional module in our project for screening, so for the adhesion module we use pSB1C3 but for enzymatic release module we use pET.


Source

For this composite part, the backbone pET of this plasmid we use is from our faculty of Health Sciences, University of Macau's laboratory reserve.

References