Coding

Part:BBa_K4716987:Design

Designed by: LIAO WENYU   Group: iGEM23_UM-Macau   (2023-10-10)


Fvp-p-6his tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1594
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 10
    Illegal BsaI site found at 355
    Illegal BsaI site found at 622
    Illegal SapI site found at 511


Design Notes

Because Fvp-p is a prolyl endopeptidase that has the effect of breaking 33-mer toxic peptide of gluten, so we use choose this kind of enzyme as one of our release enzyme in this module.


Source

The gene sequence of Fvp-p is from the gene bank of NCBI, the source: https://www.ncbi.nlm.nih.gov/nuccore/LN999508?report=genbank

References