Coding

Part:BBa_K4716986:Design

Designed by: LIAO WENYU   Group: iGEM23_UM-Macau   (2023-10-10)


PEP-6his tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1747
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 993
    Illegal XhoI site found at 1756
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1114


Design Notes

We choose to use the PEP from Aspergillus oryzae RIB40 and do our research together with Fvp-p and compare the effect of both of them to digest the 33-mer toxic peptide of gluten.


Source

This sequence we use is found from the gene bank of NCBI, the source: https://www.ncbi.nlm.nih.gov/nuccore/XM_001825944.2?report=genbank

References