Part:BBa_K4716012
pSB1C3-Pasr-mSandy
On the direction for our gene expression, there is an Pasr promoter (BBa_K4716000) which is the pH-responsive promoter native to E.coli, inducing transcription in human duodenum region’s relatively acidic environment (pH 5~6), RBS (BBa_B0034) which is the ribosome binding site, mSandy gene is our fluorescent reporter gene, double terminator (BBa_B0015) is the terminator, BioBrick prefix and BioBrick suffix are presented at the beginning and end of the whole insert gene. In the opposite direction, the cat promoter controls the CmR gene which is the selective marker, carrying chloramphenicaol resistance gene for our screening object. Followed by Lambda T0 terminator, there is also a replication origin for the whole plasmid. This plasmid is the design for validating whether or not the new fluorescent reporter gene mSandy2 we use has a good RFP property in our project replace the mCherry part of BBa_K4357004 (pSB1C3-Pasr-mCherry).
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 2049
Illegal suffix found in sequence at 1 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2049
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 2055 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2049
Illegal XhoI site found at 1033
Illegal XhoI site found at 1925 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 2049
Illegal suffix found in sequence at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 2049
Illegal XbaI site found at 2064
Illegal SpeI site found at 2
Illegal PstI site found at 16 - 1000COMPATIBLE WITH RFC[1000]
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