Plasmid
Part:BBa_K4716007:Design
Designed by: LIAO WENYU Group: iGEM23_UM-Macau (2023-10-10)
pSB1C3-Pasr-CsgA-Perpa-mefp5-mSandy
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 2049
Illegal suffix found in sequence at 1 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2049
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 2055 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2049
Illegal BamHI site found at 3661
Illegal XhoI site found at 1033
Illegal XhoI site found at 1925 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 2049
Illegal suffix found in sequence at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 2049
Illegal XbaI site found at 2064
Illegal SpeI site found at 2
Illegal PstI site found at 16 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We use this backbone (PSB1C3) is because it is a classic backbone to express target gene in E. coli strain and it is a high copy BioBrick assembly plasmid with a chloramphenicol resistance marker.
Source
The origin of backbone- Imported from database: Registry of Standard Biological Parts Entry: BBa_J179000 Description: pBS1C: Bacillus subtilis vector, amyE integration, CAM-resistance