Coding
nosZ

Part:BBa_K4707002

Designed by: Zenos Thoreson   Group: iGEM23_UVU-Utah   (2023-09-08)


Nitrous Oxide Reductase Coding Sequence

Nitrous Oxide Reductase (nosZ) changes nitrous oxide to dinitrogen gas. This allows for a nitrifier denitrification pathway to continue in another organism as we insert it. It works together with AMO and HAO to take nitrate from ammonia to dinitrogen gas. This sequence is meant to be expressed in the microalgae Chlamydomonas reinhardtii.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 791
    Illegal NotI site found at 1052
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 227
    Illegal NgoMIV site found at 291
    Illegal NgoMIV site found at 571
    Illegal NgoMIV site found at 585
    Illegal NgoMIV site found at 1045
    Illegal NgoMIV site found at 1254
    Illegal NgoMIV site found at 2060
  • 1000
    COMPATIBLE WITH RFC[1000]


The above pictures show confirmation of the nosZ gene being implemented into our L2 plasmid build. The fluorescence comes from a clover tag that was used to build the nosZ L1 transcriptional unit. Colonies with a successful nosZ gene glow green.

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