Coding

Part:BBa_K4707001

Designed by: Elise Bennett   Group: iGEM23_UVU-Utah   (2023-09-07)


HAO Coding Sequence

HAO is an enzyme that works to change NH2OH to nitrous oxide. This allows for a nitrifier denitrification pathway to continue in another organism as we insert it. It works together with AMO and nitrous oxide reductase to take nitrate from ammonia to dinitrogen gas. This sequence is meant to be expressed in the microalgae Chlamydomonas reinhardtii.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 345
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1150
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
//cds/enzyme
Parameters
None