Tag

Part:BBa_K4701205:Design

Designed by: Henri Sundquist   Group: iGEM23_Aalto-Helsinki   (2023-07-12)


SpyTag for T7 systems (with C-terminus linker)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

A linker with the protein sequence GSGESGSG was added to the C-terminus of SpyTag for separation between it and the protein of interest. No codon optimization was done as the sequence was otherwise defined on the nucleotide level.

Source

The nucleotide sequence was acquired from [1]. The suggested linker GSGESGSG is also from the same source.

References

[1] Keeble, A. H., & Howarth, M. (2019). Insider information on successful covalent protein coupling with help from SpyBank. In Methods in Enzymology (Vol. 617, pp. 443–461). Academic Press Inc. https://doi.org/10.1016/bs.mie.2018.12.010