Composite

Part:BBa_K4695220:Design

Designed by: Maciej Małolepszy   Group: iGEM23_IFB-Gdansk   (2023-10-03)


dcx1-docxylY device


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 205
    Illegal EcoRI site found at 538
    Illegal EcoRI site found at 604
    Illegal XbaI site found at 96
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 205
    Illegal EcoRI site found at 538
    Illegal EcoRI site found at 604
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 205
    Illegal EcoRI site found at 538
    Illegal EcoRI site found at 604
    Illegal BglII site found at 30
    Illegal BamHI site found at 977
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 205
    Illegal EcoRI site found at 538
    Illegal EcoRI site found at 604
    Illegal XbaI site found at 96
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 205
    Illegal EcoRI site found at 538
    Illegal EcoRI site found at 604
    Illegal XbaI site found at 96
    Illegal AgeI site found at 415
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This device uses:

  • AB_T7_lacO promoter (BBa_J435350)
  • BC_BCD23 RBS (BBa_J435385)
  • EF_T7term terminator (BBa_J435361)

All non-coding parts and linkers were chosen from the E. coli Protein Expression Toolkit collection.
This device is compatible with AF vectors.
FLANKED 5' fusion site: GGAG, 3' fusion site: CGCT.

Source

Dcx1:
Organism: Pantoea dispersa BJQ0007
Article: https://doi.org/10.1016/j.jfca.2021.104201

DocXylY:
Organism: Acetovibrio Thermocellus
Structure: https://www.rcsb.org/structure/1OHZ

Registry of standard biological parts

References