Composite

Part:BBa_K4695130:Design

Designed by: Maciej Małolepszy   Group: iGEM23_IFB-Gdansk   (2023-10-03)


scfL-his


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 589
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 768
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1659
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This composite part consists of:

  • ScfL coding sequence (BBa_K4695030)
  • DT4_GS flexible linker (BBa_J435309)
  • T4T5_GS flexible linker (BBa_J435349)
  • C-terminal T5E_6H HisTag linker (BBa_J435369)

FLANKED 5' fusion site: AATG, 3' fusion site: GCTT.
Source sequences were optimized and checked for BsaI and SapI restriction sites using: http://genomes.urv.es/OPTIMIZER/

Source

ScfL:
Cellulose binding domain:
Organism: Acetovibrio thermocellus
Structure: https://www.uniprot.org/uniprotkb/Q06851/entry

Cohesin 1:
Organism: Acetovibrio thermocellus
Structure: https://www.rcsb.org/structure/1OHZ

Cohesin 2:
Organism: Acetivibrio cellulyticus
Structure: https://www.rcsb.org/structure/4UYQ

Cohesin 3:
Organism: Bacteroides cellulosolvens
Structure: https://www.rcsb.org/structure/2Y3N

Registry of standard biological parts

References