Coding

Part:BBa_K4695020:Design

Designed by: Maciej Małolepszy   Group: iGEM23_IFB-Gdansk   (2023-10-01)


dcx1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 13
    Illegal EcoRI site found at 346
    Illegal EcoRI site found at 412
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 13
    Illegal EcoRI site found at 346
    Illegal EcoRI site found at 412
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 13
    Illegal EcoRI site found at 346
    Illegal EcoRI site found at 412
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 13
    Illegal EcoRI site found at 346
    Illegal EcoRI site found at 412
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 13
    Illegal EcoRI site found at 346
    Illegal EcoRI site found at 412
    Illegal AgeI site found at 223
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

FLANKED 5' fusion site: AATG, 3' fusion site: AGGT.
Source sequence was optimized and checked for BsaI and SapI restriction sites using: http://genomes.urv.es/OPTIMIZER/

This part has a START codon at the 5' end of the sequence and no STOP codon at the 3' end because it was designed as a part of a fusion protein with interchangable domains. If you want to express only this coding sequence, please add the STOP codon in your design.

Source

Organism: Pantoea dispersa BJQ0007
Article: https://doi.org/10.1016/j.jfca.2021.104201

References