Part:BBa_K4693010:Design

miRNA 124 nanoprobe sequence B

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Ensure that the looped region is completely complementary to miRNA 124, and the rest of the sequence should be kept constant. Ensure that the quencher is compatible with the fluorophore used in the probe.

Source

Tian H, Yuan C, Liu Y, Li Z, Xia K, Li M, Xie F, Chen Q, Chen M, Fu W, Zhang Y. A novel quantification platform for point-of-care testing of circulating MicroRNAs based on allosteric spherical nanoprobe. J Nanobiotechnology. 2020 Oct 31;18(1):158. doi: 10.1186/s12951-020-00717-z. PMID: 33129342; PMCID: PMC7603675.

Modified by iGEM IISER-TVM

References

Tian H, Yuan C, Liu Y, Li Z, Xia K, Li M, Xie F, Chen Q, Chen M, Fu W, Zhang Y. A novel quantification platform for point-of-care testing of circulating MicroRNAs based on allosteric spherical nanoprobe. J Nanobiotechnology. 2020 Oct 31;18(1):158. doi: 10.1186/s12951-020-00717-z. PMID: 33129342; PMCID: PMC7603675.