DNA

Part:BBa_K4693004:Design

Designed by: Vibhavari Medepalli   Group: iGEM23_IISER-TVM   (2023-10-10)


cDNA Cortisol 1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 31
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Towards the 3' end of the DNA sequence, we added a quencher modification, BHQ1, which has an absorption range from 480 to 580nm with maximum absorption at 534nm. Therefore, it would be able to successfully absorb the energy from the aptamer's FAM fluorophore, effectively creating our FRET system.



Source

This was designed by iGEM IISER-TVM 2023.

References