DNA

Part:BBa_K4693000:Design

Designed by: Aabha P Shams   Group: iGEM23_IISER-TVM   (2023-10-09)


Serotonin 44-mer aptamer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We needed to change the DNA code of the reverse-translated protein sequences from UniProt for E. coli, and add spacer sequences at the beginning and end so that we could add our restriction sites Nde1 and Bamh1.


Source

We got the protein sequence of this coding region from uniprot: https://www.uniprot.org/uniprotkb/P63092/entry

References