Coding

Part:BBa_K4692000:Design

Designed by: Shamlan Reshamwala   Group: iGEM23_ICT-Mumbai   (2023-10-10)


Phytase from Escherichia coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The DNA sequence has been optimized for expression in the yeast Saccharomyces cerevisiae. The native secretion signal has been replaced with the alpha mating factor sequence from Saccharomyces cerevisiae. A GS linker has been added between the alpha mating factor sequence and the coding sequence.

Source

This gene is from Escherichia coli MG1655 (EcoCyc accession EG10033).

References

https://ecocyc.org/gene?orgid=ECOLI&id=GLUCOSE-1-PHOSPHAT-MONOMER