Part:BBa_K4689619

Ribulose bisphosphate carboxylase large chain

RuBisCO is a complex enzyme found in the chloroplasts of plant cells, cyanobacteria, and some other photosynthetic organisms. Its core catalytic subunit is encoded by the rbcL gene. This enzyme is responsible for two primary reactions: Carboxylation: RuBisCO catalyzes the carboxylation of ribulose-1,5-bisphosphate (RuBP) by combining it with carbon dioxide (CO2) from the atmosphere. This reaction yields two molecules of 3-phosphoglycerate (3-PGA). Oxygenation: In addition to its carboxylation activity, RuBisCO can inadvertently oxygenate RuBP, leading to the formation of one molecule of 3-PGA and one molecule of a two-carbon compound, phosphoglycolate. This reaction is part of a process called photorespiration and is considered wasteful as it does not contribute to carbon fixation. Significance in the Calvin Cycle: RuBisCO's significance in the Calvin cycle is paramount: Carbon Fixation: RuBisCO initiates the Calvin cycle by capturing atmospheric CO2 and incorporating it into organic molecules. This process is the foundation of carbon fixation, as it converts a gaseous form of carbon into a biologically useful, organic compound, 3-PGA. Generation of Organic Compounds: The 3-PGA molecules generated by RuBisCO serve as the starting point for the synthesis of sugars and other organic compounds. Through a series of enzymatic reactions, these compounds are further processed and transformed, providing energy and carbon skeletons for the plant's growth and metabolism. Regeneration of RuBP: RuBisCO's action also generates 3-PGA, which, through several enzymatic steps, can be converted back into RuBP. This regeneration is crucial because RuBP is required for the continual operation of the Calvin cycle. Ensuring a steady supply of RuBP is essential for maintaining efficient carbon fixation.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 178
  Illegal AgeI site found at 685
  
• 1000
  COMPATIBLE WITH RFC[1000]

References:

1.Lakshmi, N. M., Binod, P., Sindhu, R., Awasthi, M. K., & Pandey, A. (2021). Microbial engineering for the production of isobutanol: current status and future directions. Bioengineered, 12(2), 12308-12321.

2.Sherkhanov, S., Korman, T. P., Chan, S., Faham, S., Liu, H., Sawaya, M. R., ... & Bowie, J. U. (2020). Isobutanol production freed from biological limits using synthetic biochemistry. Nature communications, 11(1), 4292.