Composite

Part:BBa_K4688005:Design

Designed by: Xiaoxin Xuan   Group: iGEM23_FZPt-CHINA   (2023-10-06)


FUCO1-GFP-expression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
    Illegal BamHI site found at 3357
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1989
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1395
    Illegal BsaI.rc site found at 2072


Design Notes

Codon optimization and gene expression efficiency.


Source

FUCO1 comes from chromosome 19 of the Phaeodactylum tricornutum genome.GFP comes from aequoreavictoria.

References