Part:BBa_K4687050:Design

T2 terminator

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

CRISPR-MAD7 nuclease has been described to target a wider range of PAM sequences, i.e. 5'-YTTN-3', and has shown high gene editing activity in microbial systems with small molecular weights and a very wide range of applications. In order to make CRISPR-MAD7 nuclease can be expressed correctly and play the role of gene editing in Corynebacterium glutamicum, so it is necessary to design T2 terminator to ensure that it can be expressed correctly during the transcription and translation of CRISPR-MAD7 nuclease, and better gene gene editing, so as to improve the efficiency of gene editing.

Source

Transcription terminator T2 from the E. coli rrnB gene.