Composite

Part:BBa_K4687024:Design

Designed by: Yiming Jiang   Group: iGEM23_HBUT-China   (2023-10-02)


CRISPR-MAD7 nuclease+recE/T recombinase:MADE/T


Assembly Compatibility:
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    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1978
    Illegal EcoRI site found at 6493
    Illegal EcoRI site found at 6817
    Illegal PstI site found at 3345
    Illegal PstI site found at 4633
    Illegal PstI site found at 5194
    Illegal PstI site found at 6040
    Illegal PstI site found at 6382
    Illegal PstI site found at 7168
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    INCOMPATIBLE WITH RFC[21]
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    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1978
    Illegal EcoRI site found at 6493
    Illegal EcoRI site found at 6817
    Illegal PstI site found at 3345
    Illegal PstI site found at 4633
    Illegal PstI site found at 5194
    Illegal PstI site found at 6040
    Illegal PstI site found at 6382
    Illegal PstI site found at 7168
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    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1978
    Illegal EcoRI site found at 6493
    Illegal EcoRI site found at 6817
    Illegal PstI site found at 3345
    Illegal PstI site found at 4633
    Illegal PstI site found at 5194
    Illegal PstI site found at 6040
    Illegal PstI site found at 6382
    Illegal PstI site found at 7168
    Illegal AgeI site found at 417
    Illegal AgeI site found at 5416
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    COMPATIBLE WITH RFC[1000]


Design Notes

Integrating the recE/T system into the genome of Corynebacterium glutamicum can ensure that the system will not be lost after passage, preparing for subsequent engineering design. Using plasmids containing MAD7 nuclease sequences simplifies the editing process, accelerates our experimental progress, and improves editing efficiency.


Source

CRISPR-MAD7 nuclease was identified in Eubacterium rectale. RecE/T is derived from Bacterial,Archaeal and Plant Plastid Product.

References