Coding

Part:BBa_K4687000:Design

Designed by: Yiming Jiang   Group: iGEM23_HBUT-China   (2023-09-30)


CRISPR-MAD7 nuclease


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 650
    Illegal BglII site found at 698
    Illegal BglII site found at 1019
    Illegal BglII site found at 2234
    Illegal BglII site found at 2886
    Illegal BglII site found at 3740
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1801
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The CRISPR-MAD7 naturally employs a single RNA species to guide it to the target DNA sequence and it creates DNA DSB with sticky ends rather than blunt ends. The CRISPR-MAD7 nuclease has been described to target a wider range of PAM sequences, namely 5'-YTTN-3', and to exhibit high gene editing activity in microbial systems, with its smaller molecular weight, CRISPR-MAD7 can be used for multiple gene editing.


Source

CRISPR-MAD7 is a nuclease that belongs to the Class 2 type V-A CRISPR family (Cas12a-like). It was identified in Eubacterium rectale. It was publicly released as a nuclease freely available for both academic and commercial use.

References