Plasmid

Part:BBa_K4686053:Design

Designed by: Maia Weatherly   Group: iGEM23_Princeton   (2023-10-10)


pPB_SFFV_TfR-CTagNB_P2A_FR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This plasmid design considered Gibson Assembly in order to be made. We also considered possible adhesion pairs for microscopy experiments and how we could sort for transfected cells, which we did with the P2A_FR sequence.


Source

The primers used were designed by a member of our team.

pBR70 was given to us by a mentor. She assembled it from TfRΔEctoR1-mRFP-GFPnb was a gift from Jeanne Stachowiak (Addgene plasmid # 113555 ; http://n2t.net/addgene:113555 ; RRID:Addgene_113555) and the HeraBiolabs Piggybac vector (https://www.herabiolabs.com/piggybac-products/)

pDSG312 was a gift from Ingmar Riedel-Kruse (Addgene plasmid # 115613 ; http://n2t.net/addgene:115613 ; RRID:Addgene_115613)

References