Plasmid
Part:BBa_K4686053:Design
Designed by: Maia Weatherly Group: iGEM23_Princeton (2023-10-10)
pPB_SFFV_TfR-CTagNB_P2A_FR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This plasmid design considered Gibson Assembly in order to be made. We also considered possible adhesion pairs for microscopy experiments and how we could sort for transfected cells, which we did with the P2A_FR sequence.
Source
The primers used were designed by a member of our team.
pBR70 was given to us by a mentor. She assembled it from TfRΔEctoR1-mRFP-GFPnb was a gift from Jeanne Stachowiak (Addgene plasmid # 113555 ; http://n2t.net/addgene:113555 ; RRID:Addgene_113555) and the HeraBiolabs Piggybac vector (https://www.herabiolabs.com/piggybac-products/)
pDSG312 was a gift from Ingmar Riedel-Kruse (Addgene plasmid # 115613 ; http://n2t.net/addgene:115613 ; RRID:Addgene_115613)