Coding

Part:BBa_K4685040

Designed by: Kasandra Nicole Balzaretti   Group: iGEM23_UNILausanne   (2023-10-11)


CsgA fused with polyethylene specific peptide sequence by a linker

This composite part is composed of the CsgA(BBa_K1583000) fused with a peptide capable of binding polyethylene(BBa_K4685010).

To increase the curli fiber capacity to capture microplastic, we planned to fuse these peptide sequences to the CsgA gene via a short flexible linker.

The peptides are short enough that they do not disturb the proper folding of the curli fibers. The idea of this design is that the peptide stretches out of the beta-sheet structure of the curli fibers and has the possibility to interact with the external environment.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 473
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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