Part:BBa_K4685021

CsgA-SpyTag complex

This composite part, includes the gene for the production of the csgA (main subunit of the Curli fiber) fused to the SpyTag protein. The CsgA-SpyTag complex is capable of interacting with the Rubber-binding protein (RB) fused to the SpyCatcher protein. The SpyTag/SpyCatcher system allows an irreversible conjugation of proteins:the peptide SpyTag reacts with the protein SpyCacther and forms an intramolecular isopeptide bond between the two proteins. We decided to leverage this key-lock mechanism by combining the SpyCatcher with the Rubber-binding protein and the SpyTag with the csgA subunit. Upon reaction between the two parts, the Curli fibers would be decorated with the Rubber-binding domain

Figure 1: Schematic representation of the SpyCatcher/SpyTag system applied to our project. The SpyTag protein, located on the surface of Curli fiber, will interact with the SpyCatcher protein that is bound to the Rubber-binding protein.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]